Catalog Products » CD40 Ligand/TNFSF5 Trimer, His & Flag, Human/Cynomolgus/Rhesus macaque
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CD40 Ligand/TNFSF5 Trimer, His & Flag, Human/Cynomolgus/Rhesus macaque

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
$340.00
Z04204-100

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Product Introduction
Species Human/Cynomolgus/Rhesus macaque
Protein Construction
His Flag CD40 Ligand/TNFSF5 Trimer (Met113-Leu261)
Accession # P29965(Human) / G7Q1T4(Cynomolgus) / P63304(Rhesus macaque)
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 52.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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CD40 Ligand/TNFSF5 Trimer, His & Flag, Human/Cynomolgus/Rhesus Macaque

Immobilized CD40 Ligand/TNFSF5 Trimer, His & Flag, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 59.9ng/ml determined by ELISA. »

CD40 Ligand/TNFSF5 Trimer, His & Flag, Human/Cynomolgus/Rhesus Macaque

The purity of CD40 Ligand/TNFSF5 Trimer, His & Flag, Human is greater than 95% as determined by SEC-HPLC. »

CD40 Ligand/TNFSF5 Trimer, His & Flag, Human/Cynomolgus/Rhesus Macaque

CD40 Ligand/TNFSF5 Trimer, His & Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.