Catalog Products » CD40 Ligand/TNFSF5 Trimer hFc Chimera, Mouse
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CD40 Ligand/TNFSF5 Trimer hFc Chimera, Mouse

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
$320.00
Z04318-100

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Product Introduction
Species Mouse
Protein Construction
hFc CD40 Ligand/TNFSF5 Trimer (Met112-Leu260)
Accession # P27548
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 75.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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CD40 Ligand/TNFSF5 Trimer HFc Chimera, Mouse

Immobilized Mouse CD40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD40 Ligand/TNFSF5 Trimer hFc Chimera, Mouse, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. »

CD40 Ligand/TNFSF5 Trimer HFc Chimera, Mouse

The purity of CD40 Ligand/TNFSF5 Trimer hFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC. »

CD40 Ligand/TNFSF5 Trimer HFc Chimera, Mouse

CD40 Ligand/TNFSF5 Trimer hFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.