CD73/NT5E, His, Mouse

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.

Price	$340.00
Cat. No.	Z04327-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Mouse

Protein Construction

CD73/NT5E (Trp29-Lys549) Accession # Q61503	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. Measured by its ability to hydrolyze the 5'phosphate group from the substrate adenosine5'monophosphate (AMP). Test result meets the standard.

Expression System

HEK293

Theoretical Molecular Weight

58.6 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized CD73/NT5E, His, Mouse, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. »

The purity of CD73/NT5E, His, Mouse is greater than 95% as determined by SEC-HPLC. »

CD73/NT5E, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.