IL-1 alpha/IL-1A[Biotin], His & Avi, Human

The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.

Price	$660.00
Cat. No.	Z04486-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IL-1 alpha/IL-1A (Ser113-Ala271) Accession # P01583	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Human IL1R1, No Tag immobilized on CM5 Chip can bind IL-1 alpha/IL-1A[Biotin], His & Avi, Human in SPR assay (Biacore T200). Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

20.95 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 25-32 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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The purity of IL-1 alpha/IL-1A[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

IL-1 alpha/IL-1A[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Human IL-1R1, No Tag immobilized on CM5 Chip can bind IL-1 alpha/IL-1A[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 1.01 μM as determined in SPR assay (Biacore T200). »

Background

Target Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Synonyms	Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.