IL-1 Beta/IL-1F2[Biotin], His & Avi, Human

Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.

Price	$840.00
Cat. No.	Z04487-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IL-1 Beta/IL-1F2 (Ala117-Ser269) Accession # P01584	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

E.coil

Theoretical Molecular Weight

20.1 kDa

Apparent Molecular Weight

The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human IL-1 RII, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for IL-1 Beta/IL-1F2[Biotin], His & Avi, Human, His-Avi Tag with the EC50 of 7.8ng/ml determined by ELISA. »

The purity of IL-1 Beta/IL-1F2[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

IL-1 Beta/IL-1F2[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Synonyms	Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.