IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

Price	$840.00
Cat. No.	Z04510-100
Size	100 μg 500 μg Buy in bulk
Quantity
Est. Delivery


Ask us a question

Product Introduction Background Bottom Notes

Product Introduction

Species

Human&Mouse

Protein Construction

IL-23 alpha & IL-12 beta [Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta)] Accession # Q9NPF7 (Human IL-23 alpha) & P43432-1 (Mouse IL-12 beta)	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Back

Immobilized IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 37.1 ng/ml determined by ELISA. »

The purity of IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse is greater than 95% as determined by SEC-HPLC. »

IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Synonyms	IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.