Transferrin R/CD71[Biotin], His & Avi, Human

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Price	$660.00
Cat. No.	Z04635-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

Avi	His	Transferrin R/CD71 (Cys89-Phe760) Accession # P02786
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

77.9 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 78-95 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 200 mM Larginine(pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human Transferrin, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Transferrin R/CD71[Biotin], His & Avi, Human, His Tag with the EC50 of 0.69 μg/ml determined by ELISA. »

Transferrin R/CD71[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Immobilized Transferrin R/CD71[Biotin], His & Avi, Human, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. »

Background

Target Background	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Synonyms	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.