PVRIG hFc Chimera[Biotin], Avi, Mouse

Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.

Price	$660.00
Cat. No.	Z04679-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Mouse

Protein Construction

PVRIG (Ser35-Asp165) Accession # A0A1B0GS01	hFc	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

42.7 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Mouse Nectin-2, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for PVRIG hFc Chimera[Biotin], Avi, Mouse, hFc Tag with the EC50 of 5.04 μg/ml determined by ELISA. »

PVRIG hFc Chimera[Biotin], Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

PVRIG hFc Chimera[Biotin], Avi, Mouse, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 μM as determined in SPR assay (Biacore T200). »

Background

Target Background	Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.
Synonyms	C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.