GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Rat

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Price	$840.00
Cat. No.	Z04685-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Rat

Protein Construction

GARP & Latent TGF Beta 1 Complex [Ile18-Asn628 (GARP) & Leu30-Arg278 (Latent TGF Beta 1)] Accession # D3ZVD5 (GARP) & P17246 (Latent TGF Beta 1)	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human ITGAV&ITGB6, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Rat, His Tag with the EC50 of 0.43 μg/ml determined by ELISA. »

The purity of GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Rat is greater than 95% as determined by SEC-HPLC. »

GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Synonyms	LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.