B7-H7/HHLA2, His, Cynomolgus

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Price	$340.00
Cat. No.	Z04743-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Cynomolgus

Protein Construction

B7-H7/HHLA2 (Ile21-Asn345) Accession # XP_005548285.2	His
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Loaded B7-H7/HHLA2, His, Cynomolgus on Anti-His-Biosensor can bind Human CD28H, hFc Tag in BLI assay (Gator® Prime). Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

38.31 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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The purity of B7-H7/HHLA2, His, Cynomolgus is greater than 95% as determined by SEC-HPLC. »

B7-H7/HHLA2, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

B7-H7/HHLA2, His, Cynomolgus, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). »

Background

Target Background	B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Synonyms	B7H7; B7-H7; HHLA2; B7 Homolog 7

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.