BTN3A1/CD277, His & Avi, Cynomolgus

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).

Price	$390.00
Cat. No.	Z04754-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Cynomolgus

Protein Construction

BTN3A1/CD277 (Gln1-Trp219) Accession # A0A330KWZ1	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

26.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized BTN3A1/CD277, His & Avi, Cynomolgus, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. »

The purity of BTN3A1/CD277, His & Avi, Cynomolgus is greater than 95% as determined by SEC-HPLC. »

BTN3A1/CD277, His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Synonyms	CD277; BTN3A1; BTF5; BT3.1; BTN3.1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.