Catalog Products » 2B4/CD244/SLAMF4 hFc Chimera, Human
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2B4/CD244/SLAMF4 hFc Chimera, Human

2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
$250.00
Z04975-100

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Product Introduction
Species Human
Protein Construction
2B4/CD244/SLAMF4 (Cys22-Arg221)
Accession # Q9BZW8-2		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 49 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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2B4/CD244/SLAMF4 HFc Chimera, Human

Immobilized Human CD48, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for 2B4/CD244/SLAMF4 hFc Chimera, Human, hFc Tag with the EC50 of 0.13 μg /ml determined by ELISA. »

2B4/CD244/SLAMF4 HFc Chimera, Human

The purity of 2B4/CD244/SLAMF4 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

2B4/CD244/SLAMF4 HFc Chimera, Human

2B4/CD244/SLAMF4 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background 2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Synonyms 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.