B7-H2/ICOSLG, His & Avi, Human

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.

Price	$210.00
Cat. No.	Z05057-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

B7-H2/ICOSLG (Asp19-Ser258) Accession # O75144	His	Avi
N-term		C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

29.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized B7-H2/ICOSLG, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. »

The purity of B7-H2/ICOSLG, His & Avi, Human is greater than 90% as determined by SEC-HPLC. »

B7-H2/ICOSLG, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind B7-H2/ICOSLG, His & Avi, Human, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200). »

Background

Target Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.