GARP&Latent TGF Beta 2 Complex, His & Avi, Human

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Price	$560.00
Cat. No.	Z05332-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

GARP & Latent TGF Beta 2 Complex [His20-Leu628 (GARP) & Leu21-Ser414 (Latent TGF Beta 2)] Accession # Q14392 (GARP) & P61812-1 (Latent TGF Beta 2)	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Expression System

HEK293

Theoretical Molecular Weight

70.3 kDa(GARP)&45.53 kDa(Latent TGF Beta 2)

Apparent Molecular Weight

Due to enzyme lysis and glycosylation, the protein migrates to 72-77 kDa(GARP)&13 kDa&46-48 kDa(Latent TGF Beta 2) based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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The purity of GARP&Latent TGF Beta 2 Complex, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

GARP&Latent TGF Beta 2 Complex, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind GARP&Latent TGF Beta 2 Complex, His & Avi, Human, His Tag with an affinity constant of 8.30 nM as determined in SPR assay (Biacore T200). »

Background

Target Background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Synonyms	Garpin; GARP; LRRC32; D11S833E; GARP&Latent TGF B; GARP&TGF B; Cetermin; G-TSF; LAP; TGF-beta-2; TGF Beta 2; GARP&Latent TGF Beta 2

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.