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GFR alpha 1, His, Human

Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
$260.00
Z05346-100

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Product Introduction
Species Human
Protein Construction
GFR alpha 1 (Asp25-Ser424)
Accession # NP_665736.1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 45.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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GFR Alpha 1, His, Human

Immobilized Human GDNF, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for GFR alpha 1, His, Human,His Tag with the EC50 of 3.6ng/ml determined by ELISA. »

GFR Alpha 1, His, Human

The purity of GFR alpha 1, His, Human is greater than 95% as determined by SEC-HPLC. »

GFR Alpha 1, His, Human

GFR alpha 1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).
Synonyms GDNFRA; GFRA1; GDNFR; TRNR1; RET1L; GDNF R alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; RET ligand 1; RETL1FLJ10561
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.