GITR Ligand/TNFSF18 Trimer, His & Flag, Human

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

Price	$340.00
Cat. No.	Z05357-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

Flag	His	GITR Ligand/TNFSF18 Trimer (Gln50-Ser177) Accession # Q9UNG2
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

47.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized GITR Ligand/TNFSF18 Trimer, His & Flag, Human, His Tag at 5 μg/ml(100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 36.3ng/ml determined by ELISA. »

The purity of GITR Ligand/TNFSF18 Trimer, His & Flag, Human is greater than 95% as determined by SEC-HPLC. »

GITR Ligand/TNFSF18 Trimer, His & Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Synonyms	TNFSF18; AITRL; TL6; GITRL; GITR Ligand

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.