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IL-25/IL-17E, His, Human

IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
$390.00
Z05487-100

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Product Introduction
Species Human
Protein Construction
IL-25/IL-17E (Tyr33-Gly177)
Accession # Q9H293-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 17.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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IL-25/IL-17E, His, Human

Immobilized IL-25/IL-17E, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 4.64 μg /ml determined by ELISA. »

IL-25/IL-17E, His, Human

The purity of IL-25/IL-17E, His, Human is greater than 95% as determined by SEC-HPLC. »

IL-25/IL-17E, His, Human

IL-25/IL-17E, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-25/IL-17E, His, Human

IL-25/IL-17E, His, Human, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200). »

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Background
Target Background IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
Synonyms IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.