IL-27RA/TCCR hFc Chimera, Human

A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.

Price	$340.00
Cat. No.	Z05489-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IL-27RA/TCCR (Gly34-Lys516) Accession # Q6UWB1	hFc
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

79.3 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS, 5mM DTT (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized IL-27RA/TCCR hFc Chimera, Human, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9 ng/ml determined by ELISA. »

IL-27RA/TCCR hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-27RA/TCCR hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). »

Background

Target Background	A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
Synonyms	IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.