IL-3, His & Avi, Human

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Price	$300.00
Cat. No.	Z05492-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

Avi	His	IL-3 (Ala20-Phe152) Accession # P08700
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

E.coli

Theoretical Molecular Weight

16.9 kDa

Apparent Molecular Weight

The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL. »

Immobilized IL-3, His & Avi, Human, His Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.2 μg/ml determined by ELISA. »

The purity of IL-3, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

IL-3, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Synonyms	IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.