IL-7R alpha/CD127 hFc Chimera, Human

Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.

Price	$280.00
Cat. No.	Z05514-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IL-7 R alpha/CD127 (Glu21-Gly236) Accession # P16871-1	hFc
N-term	C-term

Purity

> 90% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

51.6 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized IL-7R alpha/CD127 hFc Chimera, Human, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.4ng/ml determined by ELISA. »

The purity of IL-7R alpha/CD127 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

IL-7R alpha/CD127 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
Synonyms	IL-7R alpha; IL7Ra; CD127; CDW127; IL7Rα

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.