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Latent GDF-8, His, Human

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
$410.00
Z05564-100

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Product Introduction
Species Human
Protein Construction
His Latent GDF-8 (Asn24-Ser375)
Accession # O14793
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 41.19 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-40 kDa and 45-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-Arginine (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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Latent GDF-8, His, Human

Immobilized Latent GDF-8, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.7ng/ml determined by ELISA. »

Latent GDF-8, His, Human

Immobilized Latent GDF-8, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 33.7ng/ml determined by ELISA. »

Latent GDF-8, His, Human

The purity of Latent GDF-8, His, Human is greater than 95% as determined by SEC-HPLC. »

Latent GDF-8, His, Human

Latent GDF-8, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Synonyms Myostatin; GDF8; Latent GDF8; Mstn
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.