LIF, Human

Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Price	$390.00
Cat. No.	Z05579-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

LIF (Ser23-Phe202)
Accession # P15018-1

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 0.1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

19.7 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50 for this effect is 0.2-2 ng/mL. (QC Test) »

Immobilized LIF, Human, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.1 μg/ml determined by ELISA. »

The purity of LIF, Human is greater than 95% as determined by SEC-HPLC. »

LIF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Synonyms	LIF; HILDA; MLPLI; CDF; DIA

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.