NKG2A&CD94 hFc Chimera, Flag, Human

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Price	$390.00
Cat. No.	Z05683-100
Size	100 μg 500 μg Buy in bulk
Quantity
Est. Delivery


Ask us a question

Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

Flag	hFc	NKG2A&CD94 (Arg100-Ala219 (NKG2A) and Ser34-Ile179 (CD94))_x000D_ Accession # P26715-1(NKG2A)&Q13241-1(CD94)
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

17.9 kDa (CD94) and 41.1 kDa (NKG2A)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Back

Immobilized NKG2A&CD94 hFc Chimera, Flag, Human, hFc Tag (100 μl/well) on the hFc Antibody precoated plate(2 μg/ml). Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. »

The purity of NKG2A&CD94 hFc Chimera, Flag, Human is greater than 95% as determined by SEC-HPLC. »

NKG2A&CD94 hFc Chimera, Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.