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Notch 3, His, Human

Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
$390.00
Z05703-100

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Product Introduction
Species Human
Protein Construction
Notch 3 (Ala1378-Ser1640)
Accession # Q9UM47		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 30.18 kDa
Apparent Molecular Weight Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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Notch 3, His, Human

Immobilized Notch 3, His, Human, His Tag at 2 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. »

Notch 3, His, Human

The purity of Notch 3, His, Human is greater than 95% as determined by SEC-HPLC. »

Notch 3, His, Human

Notch 3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Synonyms CADASIL; Notch homolog 3; Notch-3; NOTCH3
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.