NRG1 Beta 1 hFc Chimera, Human

Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.

Price	$270.00
Cat. No.	Z05710-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

hFc	NRG1 Beta 1 (Ser2-Lys246)_x000D_ Accession # Q02297-6
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

54.00 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human Her4, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for NRG1 Beta 1 hFc Chimera, Human, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. »

Immobilized Human Her3, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for NRG1 Beta 1 hFc Chimera, Human, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. »

The purity of NRG1 Beta 1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

NRG1 Beta 1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.
Synonyms	Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.