PAI-1, His, Human

PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.

Price	$300.00
Cat. No.	Z05731-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

PAI-1 (Val24-Pro402)_x000D_ Accession # P05121-1	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its ability to inhibit uPA cleavage of a peptide substrate, NcarbobenzyloxyGlyGlyArg7amido4methylcoumarin (ZGGRAMC). The IC50 value is < 35 nM. Test result meets the standard.

Expression System

HEK293

Theoretical Molecular Weight

43.86 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5).

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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The purity of PAI-1, His, Human is greater than 95% as determined by SEC-HPLC. »

PAI-1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.
Synonyms	PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.