Catalog Products » PD-1/PDCD1 mFc Chimera, Human
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PD-1/PDCD1 mFc Chimera, Human

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
$340.00
Z05735-100

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Product Introduction
Species Human
Protein Construction
PD-1/PDCD1 (Leu25-Gln167)
Accession # Q15116-1		 mFc (IgG1)
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 41.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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PD-1/PDCD1 MFc Chimera, Human

Immobilized PD-1/PDCD1 mFc Chimera, Human, mFcTag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15 μg/ml determined by ELISA. »

PD-1/PDCD1 MFc Chimera, Human

The purity of PD-1/PDCD1 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

PD-1/PDCD1 MFc Chimera, Human

PD-1/PDCD1 mFc Chimera, Human on Bis-Tris PAGE under reduced condition.The purity is greater than 95%. »

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Background
Target Background Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed?). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.