SECTM1 hFc Chimera, Human

SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.

Price	$240.00
Cat. No.	Z05799-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

SECTM1 (Gln29-Gly145) Accession # AAH17716	hFc
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

39.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 47-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human CD7, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for SECTM1 hFc Chimera, Human, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. »

The purity of SECTM1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

SECTM1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

SECTM1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Mouse CD7, His Tag with an affinity constant of 4.46 μM as determined in SPR assay (Biacore T200). »

Background

Target Background

SECTM1 is a T/NK cell 'co-stimulatory' molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.

Synonyms

K-12; SECTM1

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.