Catalog Products » Siglec-10 (R119A), His & Avi, Human
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Siglec-10 (R119A), His & Avi, Human

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
$390.00
Z05816-100

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Product Introduction
Species Human
Protein Construction
Siglec-10 (R119A) (Met17-Thr546)_x000D_
Accession # Q96LC7-1		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per ug by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 62.64 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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Siglec-10 (R119A), His & Avi, Human

Immobilized Siglec-10 (R119A), His & Avi, Human , His Tag at 1 μg/ml(100 μl/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. »

Siglec-10 (R119A), His & Avi, Human

The purity of Siglec-10 (R119A), His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Siglec-10 (R119A), His & Avi, Human

Siglec-10 (R119A), His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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Background
Target Background Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.