TNFSF15, Fc Chimera, Human

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Price	$370.00
Cat. No.	Z05903-100
Size	100 μg 500 μg Buy in bulk
Quantity
Est. Delivery


Ask us a question

Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

monomeric Fc	TNFSF15 (Leu72-Leu251)_x000D_ Accession # O95150-1
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

44.87 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Back

Immobilized Mouse DR3, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for TNFSF15, Fc Chimera, Human, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. »

Immobilized TNFSF15, Fc Chimera, Human, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. »

The purity of TNFSF15, Fc Chimera, Human is greater than 90% as determined by SEC-HPLC. »

TNFSF15, Fc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A

Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.