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Protein A ELISA Kit

Protein A ELISA Kit

Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry. Moreover, it also probably causes false results in immunological assays if antibody product is contaminated with Protein A.

GenScript Protein A ELISA Kit is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe™ in the presence or absence of IgG antibodies. The kit offers users in the pharmaceutical industry a sensitive assay to measure mini amounts of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of the resins under specific conditions.

Key Features


Cat. No.
Product Name
Size
Price
Quantity
L00430 Protein A ELISA Kit
1 kit
$550.00
 

Components
Quantity
96-well plate coated with anti-Protein A mAb
1 plate (8 wells x 12 strips)
Biotin conjugated anti-Protein A mAb
12 ml
Streptavidin-HRP
12 ml
Protein A Standard (10 μg/ml)
1 ml
MabSelect SuRe™ Protein A Standard (10 μg/ml)
1 ml
20 × Wash Solution
40 ml
One-Solution Microwell TMB Substrate
12 ml
Stop Solution
6 ml
All components should be stored at 2-8 °C.
Protein A ELISA Kit

Typical Assay Data

Protein A ELISA Kit
Conc. of Protein A (ng/ml)
OD 450
Duplicate 1
Duplicate 2
Average
Adjusted Average
3.20
1.711
1.829
1.770
1.717
1.60
0.849
0.871
0.860
0.807
0.80
0.461
0.468
0.465
0.412
0.40
0.247
0.257
0.252
0.199
0.20
0.163
0.164
0.164
0.111
0.10
0.141
0.149
0.145
0.092
0.00
0.077
0.076
0.077
0.024
Blank
0.052
0.054
0.053

Figure 1. Standard curve generated using GenScript Protein A ELISA Kit (L00430).
 

Precision

intra-assay
inter-assay
# of tests
Meanng/ml
CV%
# of assays
Meanng/ml
CV%
12
1.6
1.60
8
1.6
3.70
12
0.4
4.05
8
0.4
5.42
12
0.1
4.56
8
0.1
7.59

Precision is defined as the percent coefficient of variation (%CV). The data above shows CV value of both intra and inter-assay for 3 control samples in low, mid and high concentration range.

Intra-assay Precision CV<5% and Inter-assay Precision CV<10%

 

Recovery


Sample Matrix
Protein A Added (ng/ml)
% Recovery
6.20 mg/ml human IgG
1.50
85.01
1.19 mg/ml customer Ab
1.00
98.07
1.13 mg/ml human IgG
1.20
108.96
1.00 mg/ml human IgG
2.50
100.01

The assay was performed by spiking the Protein A standards provided in this kit into the IgG samples. The recovery rates are within 85% to 115%.

 

Cat. No.
Product Name
Size
Price
Quantity
L00210 Protein A Resin
5 ml
$98.00
M00091 Streptavidin-HRP
1 mg
$89.00
A00728-40 Protein A Antibody, pAb, Chicken
40 μg
$99.00
A00728-100 Protein A Antibody, pAb, Chicken
100 μg
$199.00
A00729-40 Protein A Antibody [HRP], pAb, Chicken
40 μg
$99.00
A01778 Protein A Antibody, mAb, Mouse
100 μg
$199.00
A01779 Protein A Antibody [Biotin], mAb, Mouse
100 μg
$199.00
 

 
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