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Case Study—A 10-fold Increase in Protein Expression Level in E. coli System Using OptimumGeneTM Gene Design Technology


GenScript's proprietary OptimumGeneTM Codon Optimization and gene design technologies can significantly improve the expression level of target proteins. Our advanced optimization takes the entire mRNA structure into consideration as opposed to traditional optimization methodologies which only consider the codon usage frequency and the structure of mRNA partially. The patent-pending OptimumGeneTM Codon Optimization Technology can typically increase the protein expression level in E. coli by ten-fold, provided that the protein expression and purification methods are appropriate.

Figures below manifested the effectiveness of the OptimumGeneTM Codon Optimization Technology in increasing protein expression levels for two different genes expressed in E. coli.

 

Fig. A. GenScript's OptimumGeneTM codon optimization delivered 10 times higher expression level for protein α compared to non-optimized native gene sequence. The expression level was 3 times more than that from Competitor GA's optimization method.
 

Fig. B. GenScript's OptimumGeneTM codon optimization delivered 20 times higher expression level for protein β compared to non-optimized native gene sequence. The expression level was 13 times more than that from Competitor GA's optimization method.

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