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Radiometric Assay Service

Custom Receptor Binding Assay Services

Radioligand binding assay methods are preferred to study the binding properties of test compounds in drug discovery, such GPCRs and ion channels. However, the regulatory requirements, high construction and maintenance costs prohibit most of labs from utilizing an exclusive radioisotope-based assay platform.

To address the problem, GenScript has constructed a powerful radioisotope-based assay platform as a low cost solution for your project. Based on the platform, scintillation proximity assay (SPA) and filtration binding assay are available tailored to your specifications. In addition, GenScript has validated many GPCR binding assays and hERG binding assay for your immediate screening.

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  • Ready Catalog Binding Assays

Filtration Assays

   
Filtration: Saturation Binding Assay   Filtration: Competition Binding Assay
Fig 1. 10 μg of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of [3H]Prazosin in the absence (total binding) or presence of 1000-fold excess unlabeled Prazosin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
Fig 2. 10 μg of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of Phentolamine in the presence of 1nM [3H]Prazosin. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

SPA Assays

   
SPA: Saturation Binding Assay
SPA: Competition Binding Assay
Fig 3. 10 μg of membranes prepared from HEK293 cells stably expressing 5-HT2C receptors were incubated with 0.5 mg of WGA SPA beads and increasing concentrations of [3H]5-HT in the absence (total binding) or presence of 1000-fold excess unlabeled 5-HT (nonspecific binding, NSB) overnight at room temperature. Radioactivity was counted on TopCount. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
Fig 4. 10 μg of membranes prepared from HEK293 cells stably expressing 5-HT2C receptors were incubated with 0.5 mg of WGA SPA beads and increasing concentrations of 5-HT at room temperature overnight in the presence of 25 nM [3H]5-HT. Radioactivity was counted on TopCount. Data were fit to one-site competition equation using a non-linear regression method.
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SC1485
Custom Receptor Binding Assay Services
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