Shotgun Library Services
Shotgun libraries are one efficient way of generating DNA sequences and of identifying specific genes from cloned DNA fragments in BAC or cosmid vectors. GenScript provides high-quality shotgun library construction and sequencing services from a variety of sources and formats including BAC, PAC, Cosmid, and Fosmid. Clones within the shotgun libraries can be tailored to carry inserts within a narrow size range. Our final delivery includes transformed cells or frozen subclones arrayed on 96-well plates, ready to prep and sequence.
Key Features:
- Quality Template: GenScript's quality DNA preparation ensures a high-quality library template.
- Efficient Cloning: The narrow, controlled size range of the inserts improves cloning efficiency.
- Large-Scale Sequencing: GenScript's large-scale and high-throughput DNA sequencing capabilities facilitate library sequencing.
- Cloning of Difficult Regions: GenScript has extensive experience in cloning so-called unclonable DNA regions, such as AT- and GC-rich DNA, repeats, and hairpins.
Service Specifications:
GenScript offers BAC, PAC, Cosmid, and Fosmid shotgun libraries and library sequencing services.
1. Library Construction:
- Plasmid DNA purification and quality analysis
- Shotgun library construction with randomly sheared DNA inserts of 1.5-3 kb
- Subcloning of DNA fragments into GenScript's pLY cloning vectors
- PCR screening of the clones containing inserts
- Plasmid purification of selected clones
- Transformation and glycerol stock production
- Assembly of overlapping sequences
2. Library Sequencing:
- Plating, picking, and arraying colonies into 96-well format
- High-throughput sequencing on ABI 3730xl DNA sequencers
- Contiguous alignment and assembly
- Up to 99% coverage
3. Library Quality Control:
- Less than 5% E. coli genomic DNA contamination
- 1.5-3 kb inserts
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Delivery Specifications:
The final delivery includes either transformed cells or frozen cultures of subclones arrayed on 96-well plates, ready for DNA prep and sequencing. Upon request, GenScript can provide DNA extraction and sequencing services and subclone trimming and assembly services.
- Shotgun libraries of individual clones or plasmids with small DNA inserts
- QA/QC data including sequencing data of 96 clones
Optional Services:
- Assembled DNA sequences up to full coverage
- Publication-ready DNA sequences (using ABI PrismTM 3730xl sequencers)
- Electropherograms of each run
- Subclones, plasmid DNAs, and internal primers
- Additional clones are available upon request
Quotations and Ordering:
You may contact us for quotation requests by phone or fax listed below. However, we recommend that you send your quotation requests via our secure online messaging system or by email at gene@genscript.com. Our customer service representatives are available 24 hours, Monday through Friday to assist you. In the body of the message, please include the following:
- Relevant details regarding the starting clone, including vector background and insert size
- Desired insert size of subclones
- Detailed service requirements including desired delivery specifications
Please submit your plasmid DNA or stab cultures of the respective clones with detailed project requirements to Shotgun Library Construction, GenScript Corp., 120 Centennial Ave., Suite 105, Piscataway, NJ08854, US.
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Order by email: gene@genscript.com |
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Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Order by fax: 1-732-210-0262 1-732-885-5878 |
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