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Inducible siRNA Expression Vectors

The H1.2 promoter is an engineered inducible promoter** containing a tetracycline operator (TetO1). The tetracycline operator itself has no effect on expression. When the tetracycline repressor (TetR) is present, it effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR, causing the TetR protein to release the TetO1 site, derepressing transcription.

pRNATin-H1.2 is designed for mammalian transfection. Each vector carries a neomycin or hygromycin resistance gene, which can be used to establish stable cell lines, and a coral GFP marker under CMV promoter* control, which can be used to track transfection efficiency. Adenoviral, retroviral, and lentiviral expression vectors are also available for this inducible system.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1223
H1
Neo
cGFP
Map
$350
SD1224
H1
Hygro
cGFP
Map
$350
SD1220
H1
Neo
-
Map
$350
SD1229
H1
Kanamycin
cGFP
Map
$350
SD1234
H1
Neomycin
-
Map
$350
SD1254
H1 (inducible)
Hygro
cGFP
Map
$350
SD1260
H1 (inducible)
Neo
cGFP
Map
$350

We have designed a positive control for this inducible expression vector, SD1523 (pRNATin-H1.2/Neo/siFLuc), that can knock down firefly luciferase.

* Limited Use Label License: The use of CMV promoter is covered under U. S. Patents Nos. 5,168,062 and 5,385,839, owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF at 319-335-4546.

** Limited Use Label License: This H1.2 inducible promoter is covered by United States Patent Applications Nos. 60,505,677, owned by GenScript Corporation. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) under these patent rights to perform the siRNA synthesis methods claimed in the patent application for research and development purposes solely in conjunction with this product.