Stable Cell Line Services for Assay Development (SC1394)
In vitro assays based on gene engineered cells play crucial roles in modern drug discovery processes. Especially for GPCRs and ion channels, multiple trans-membrane proteins, the cell-based assays are dominant in chemical library screening. There are many stable cell lines and assay techniques are developed to model human bodies. With expertise on cell line generation and assay development, GenScript offers your Gene-to-Assay solution for your drug discovery project.
GenScript has developed a Ready-to-Assay GPCR & Ion Channel Cell Line Collection which is available as product for your order. If you can't find the stable cell lines of your interests, please choose the service to customize your stable cell lines with our available assays or other assays upon your specifications.
GenScript also offers a lentiviral based service, CellPower™, to generate a stable cell line with any cell and any gene
Flexible Solutions for Any Target
|Any cell line (Amaxa® Nucleofector® technology from Lonza) || |
WB or FACS
|Radioisotope-based ||Fluorescence-based, luminescence-based, or electrophysiology-based |
GenScript utilizes 2-round selection strategy for GPCR and ion channel stable cell line generation upon different applications. To reduce the cost and timeline, GenScript chooses HTS-compatible fluorescence- or luminescence-based assays to screen stable clones in the 1st round selection. In 2nd round selection, radioisotope- and electrophysiology-based assays are available upon your applications. For nuclear receptors, transporters, and other targets which have no ready HTS-compatible assays, GenScript offers mRNA or protein level assays as alternative solutions.
In addition, GenScript can develop a new assay upon your specifications. Please feel free to contact us for technical support.
Note: CHO-K1, CHO-K1/Gα15, CHO-K1/Gqi5, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, and U-2OS are available as starting cell lines. For special requirements, please feel free to contact us.
1st Round Selection
2nd Round Selection
|GPCRs ||Gq-coupled ||Calcium assay ||Radioligand binding assay |
|Gi/Gs-coupled ||Calcium assay (Gqi5/Gα15), cAMP assay, or luciferase reporter gene assay |
|Ion Channels ||Calcium channels ||Calcium assay ||Radioligand binding assay or manual patch clamp assay |
|Potassium channels ||Thallium flux assay or membrane potential assay |
|Sodium channels ||Membrane potential assay |
|Chloride channels ||YFP reporter gene assay |
Nuclear Receptors, Transporters, etc.
| ||Upon client's specifications ||Upon client's specifications |
Quotations and Ordering
To request a quotation, please download and complete the Quotation/Order Form and send it back by email or fax.
To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our Secure Online Messaging System.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.