Mammalian Stable Cell Line Development
The pharmaceutical industry uses stable cell lines in target discovery, target validation, assay development, and compound screening. They are also used in academia to evaluate the mechanisms of biological processes.
The generation of stable cell lines is a time-consuming and expensive process. GenScript offers a collection of stable mammalian cell lines that express a variety of proteins including members of the G protein-coupled receptor family (GPCRs), ion channels, kinases, and other enzymes. In addition, we offer a number of specialized host cell lines incorporating various reporter assay systems for the development of GPCR, ion channel, and kinase assays.
GenScript also provides custom services in the generation of stable cell lines for our customers' genes of interest. Our Mammalian Stable Cell Line Development Services not only saves you time but also takes full advantage of our significant expertise in gene synthesis, cloning, stable cell line creation, and assay development.
We guarantee:
We will provide two free replacements (1 vial for each replacement ) within a 2-year period if you accidently lose your cell line.
Key Features:
- Screening and research-ready GPCR stable cell lines
- Custom construction of any other GPCR cell line
- Ready-to-Go frozen cells ready for direct use in your assays the next day
- Proprietary transient transfection system gives fast-turnaround GPCR-expressing mammalian cells
- Rodent homologous receptors or proteins in a few days
- You name a GPCR, and we will construct an expression system for you
- Competitive pricing for all stable and transient expression cells
- GPCR Cell Lines
- Host Cell Lines
- Custom Services
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HEK293/4xCRE/luciferase
CHO-K1/4xCRE/luciferase
HEK293/PTHR1/CRE/beta-lactamase
HEK293/PTHR2/CRE/beta-lactamase
HEK293/4xCRE/beta-lactamase
CHO-K1/4xCRE/beta-lactamase
HEK293/CRE/d2EGFP
HEK293/CTR/CRE/d2EGFP
HEK293/PTHR/CRE/d2EGFP
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HEK293/3xNFAT/beta-lactamase
CHO-K1/3xNFAT/beta-lactamase
HEK293/PAFR/NFAT/beta-lactamase
HEK293/3xNFAT/luciferase
CHO-K1/3xNFAT/luciferase
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HEK293/Ga15
HEK293/Ga16
HEK/pLDLR270/Luc
HepG2/pLDLR270/Luc
HEK293/SPR
HEK/5xSRE/Luc
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Step description |
Required Time (weeks) |
| 1. Gene synthesis (optional) |
2 |
| 2. Cloning of your gene into an expression vector (optional) |
2-4 |
| 3. Stable transfection |
1 |
| 4. Selection of positive clones |
3-5 |
| 5. Positive clone identification and characterization* |
4-6 |
Total |
12-18 |
* Upon request positive clone characterization may include dose
response curves for agonists/antagonists, stability tests, assay
optimization, Z-factors for HTS, mycoplasma tests and other assays.
Delivery Specifications:
- 3 highest expressing clones (2-3 ml of 106 cells/ml)
- Associated
documentation (vector maps, all protocols, reports for all QC tests,
assay SOPs and validation data, HTS validation if applicable)
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Quotations and Ordering:
 To request a quote, please download, complete and email the Quote Request Form.
Orders can be placed by email, phone or fax either a PO (Purchase Order) or credit card. For email or fax orders, please download, complete and email the Order Form.
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Email: bioassay@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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