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Non-viral Stable Cell Line Services
Stable Cell Line Services Quotation

Non-viral Stable Cell Line Services for Assay Development

In vitro assays based on stable cell lines play a crucial role in modern drug discovery processes. When high expression of the target gene is important for assay development, GenScript's non-viral stable cell line service can deliver the stable cell pool or a single stable clone. GenScript also has the capability to test the function of the gene of interest in our in vitro assays.

GenScript offers a comprehensive range of cell line engineering, including both lentiviral and non-viral stable cell line services, and GenCRISPR™, CRISPR based knock-out or knock-in stable cell line services. We have a proven track record of success in stable cell line generation and assay development, offering clients a gene-to-assay solution for drug discovery.

In addition to offering a custom stable cell line service, GenScript also has a product catalog of over 170 GPCR & ion channel cell lines, ready to assay.

Workflow of Standard Non-viral Stable Cell Line Development

Workflow of Fast Stable Cell Line Development Process

Service Packages Deliverables Turnaround time Validation/QC Price
Standard non-viral cell line development (lipofection or Amaxa® Nucleofector® technology based transfection)  2 validated single cell clones (2 vials per clone)  
~23 weeks
  • mRNA level – qPCR analysis
  • Protein level – Western blot or FACS analysis
Please quote
Fast stable cell line development (lipofection based transfection only) 
  • Adherent cell lines – two vials of stable pools at 1x106 cells/vial
  • Suspension cell lines – two vials of stable pools at 1x107 cells/vial
5-7 weeks
Protein level- FACS analysis 

Note: CHO-K1, CHO-K1/Gα15, CHO-K1/Gqi5, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, and U-2OS are available as starting cell lines. For special requirements, please feel free to contact us.

Additional Services 
4 or 16 passage stability test  Membrane preparation 
Expansion and cryo-preservation  Functional assay development including: fluorescence-based and radioligand-based binding assays, manual patch clamp and cell-based Westerns

Related services

CellPower™ - lentiviral-based stable cell line service, ideal for difficult to transfect cell lines, and hard to express genes. Antibody and protein expression stable cell lines – Stable cell lines developed for bioproduction purposes GenCRISPR™ - CRISPR based knock out or knock in stable cell line service

Case Study

Adenosine Receptor A1 Stable Cell Line Generation

A1 receptor is a Gαi-coupled GPCR which theoretically cannot be assayed by calcium mobilization assay. We co-expressed A1 receptor with Gα15, a promiscuous Gα protein, in CHO-K1 cells and selected a best clone as the primary clone and developed calcium mobilization assay.

1. Biology of Adenosine Receptor A1

Activation of A1 receptor, a ubiquitous Gαi-coupled GPCR, elicits an inhibition of adenylate cyclase and causes decrease in cAMP concentration. Adenosine antagonists are widely used in neonatal medicine.

2. Stable Clone Screening

We transfected CHO-K1 cells with the ADORA1 gene which encodes A1 receptor and got 96 primary clones by antibiotic selection. These 96 clones were further screened for the positive clones through calcium mobilization response (Fig. 1). Based on the long-lasting signal intensity profile over several months in culture, Clone 32 and Clone 7 were selected as the primary and backup clones for CHO-K1/ADORA1/Gα15 cell line, respectively (Fig. 2). All the further studies were performed on the primary clone, Clone 32.

Selection of clonesStability test of clones
▲ Figure 1
▲ Figure 2
3. 29-Passage Stability Test

We compared EC50 values of NECA, an ADORA1 agonist, at various passages of Clone 32. The variation of EC50 values of Clone 32 from passage 1 to 29 passages was within a small window (see below). Based on the pharmacology study, our results showed our Clone 32 has a high level of passage stability. The EC50 values that we have obtained matched very well with those reported in the literature (EC50 value: 20 ~ 173 nM1,2, Tab. 1).

 Passage Number  P1  P5  P10  P15  P20  P23  P26  P29
 EC50 (n=2) nM  32±6  68±5  41±7  43±2  72±6  65±4  56±9  45±7
▲ Table 1

1. Yolande Cordeaux et al. (2004) Coupling of the human A1 adenosine receptor to different heterotrimeric G proteins: evidence for agonist-specific G protein activation. Brit. J. Pharmacol., 143: 705-714.
2. Cordeaux Y et al. (2000) Influence of Receptor Number on Functional Responses Elicited by Agonists Acting at the Human Adenosine A1 Receptor: Evidence for Signaling Pathway-Dependent Changes in Agonist Potency and Relative Intrinsic Activity. Mol Pharmacol. 58(5):1075-84

Quotations and Ordering

  • To request a quotation, please download and complete the Stable Cell Line Services Quotation and send it back by email or fax.
  • To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our Secure Online Messaging System.