CrystalProTM Gene-to-Structure Services
GenScript provides comprehensive services for structural biology, including high purity protein preparation, fragment-based crystallographic screening, crystallization and structure determination. With our proprietary OptimumGeneTM Codon Optimization Technology, comprehensive portfolio of expression systems, a wide selection of purification and crystallization methods, GenScript can handle your projects starting from gene synthesis to crystallization/co-crystallization and structure determination.  GenScript has developed advanced purification platform to provide protein samples of "crystallographic grade" purity. The purity of final products is better than 95% and can be directly used for crystallization and other high quality assays. Our extensive experience in crystallization screening and crystallization condition optimization ensures us to prepare the best quality protein crystals for structure-based researches. All crystal structures at GenScript will be determined with the data sets collected using synchrotron light sources.The quality of the final X-ray structure will match the publication requests. We have advanced techniques in obtaining co-crystal structures in complex with leads via co-crystallization or soaking method. The electron density map of ligands in a complex structure will be well refined in order to precisely determine the position of all atoms. GenScript can screen commercially available fragment libraries using X-ray crystallography, collect high-resolution synchrotron datasets and determine exact binding mode of the fragments to your protein target. Our well-established CrystalProTM Gene-to-Structure Platform allows us to provide valuable structural insights to support your lead optimization and fragment based discovery programs.
- Service Specifications
- Service Flowchart
- Technologies
CrystalProTM Gene-to-Structure Services (SC1546) Phase I: High purity protein production | Milestones | Specifications | Deliverables | | Expression verification and optimization | - Gene synthesis with codon optimization
- Subclone the gene into expression vector(s)
- Expression evaluation
- Expression optimization
| Gene in pUC57
Constructs report
Expression report | | Scale-up protein production | - Protein expression
- Multi-step purification
- QC by SDS-PAGE, Western blot
| High purity protein |
Phase II: Crystallization and structure determination | Milestones | Specifications | Deliverables | | Crystallization screening | - Crystallization Screening
- Crystallization conditions optimization
| Protein crystal | | X-ray data collection | - Collection of high-resolution X-ray data using synchrotron source
| X-ray data | | Crystal structure determination | - Structure determination with crystallography softwares
| Density map, protein structure (PDB) and statistics |
Phase III: Protein-compound complex structure determination | Milestones | Specifications | Deliverables | | Co-crystallization | - Soaking of compounds
- Co-crystallization of compounds (optional)
| Co-crystal | | X-ray data collection | - Collection of high-resolution X-ray data using synchrotron source
| X-ray data | | Crystal structure determination | - Structure determination with crystallography softwares
| Density map, protein structure (PDB) and statistics |
| High Purity Protein Preparation GenScript has developed advanced purification platform to provide protein samples of "crystallographic grade" purity. The purity of final products is better than 95% and can be directly used for crystallization and other high quality assays. Combining fermentors and large sized chromatography systems, we can deliver quantities of high purity protein samples ranging from milligrams to grams level. Fragment-based Crystallographic Screening Fragment-based drug discovery (FBDD) is a new paradigm in drug discovery that utilizes small molecules fragments from larger molecules. It is a faster, cheaper, smarter way for drug discovery. We can screen commercially available fragment libraries using X-ray crystallography, collect high-resolution synchrotron datasets and determine exact binding mode of the fragments to your protein target. Crystallization and Structure Determination 3-D structures determined by X-ray crystallography have become an integral part of the current drug discovery process. Structure based drug design has proven effective in nearly all aspects of drug discovery and development.
Our extensive experience in crystallization screening and crystallization condition optimization ensures us to prepare the best quality protein crystals for structure-based researches. All crystal structures at GenScript will be determined with the data sets collected using synchrotron light sources. The quality of the final X-ray structure will match the publication requests. Our scientists have experiences in solving various drug targets including kinases, polymerases, chaperones, membrane proteins, protein/protein complex, etc. Co-crystal Structure Determination Finding a compound in the co-structure not only depends on its affinity, but also on its solubility, specific interaction, and location of the binding site. GenScript has advanced techniques in obtaining co-crystal structures in complex with leads via co-crystallization or soaking method. The electron density map of ligands in a complex structure will be well refined in order to precisely determine the position of all atoms. | Quotations and Ordering:   To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system. If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US. For questions or to inquire about the status of your order, contact us by email, phone, fax or via our secure online messaging system. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.  | Email: protein@genscript.com |  | Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |  | Fax: 1-732-210-0262 1-732-885-5878 | |
