Synthetic Gene Variant Libraries (Protein Engineering)
In vitro molecular optimization is a very efficient means of generating mutant proteins with improved or novel properties, identifying regulatory sequences, and probing for structurally and functionally critical residues. Synthetic libraries constructed using the in vitro molecular optimization method provide one useful approach to the systematic study of protein properties, regulation, and function.
GenScript's strong expertise in de novo gene synthesis allows us to synthesize complex protein libraries without any dramatic increase in cost. Our synthetic library services include site-directed mutagenesis libraries, sequential permutation scanning libraries, and randomized and degenerated libraries.
Services:
1. Site-Directed Mutagenesis Libraries
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GenScript combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. The site-directed mutagenesis library offers a great platform for protein function and active center studies. In these libraries, any given residue can be substituted with any of other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.
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The final delivery of our site-directed mutagenesis library includes the following:
- Up to 4 ug of mutation constructs
- Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
- Separated variants
- Sequence verification information
- Statistical analysis of nucleotide distribution at the mutation sites
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2. Sequential Permutation Scanning Libraries
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Sequential permutation scanning is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning by replacing each amino acid with all 20 amino acids simultaneously. This technique provides a detailed profile of each amino acid at the position. For each codon of interest, a small, site-saturated library is constructed. This library can be delivered as a pool or in a separated format for any substitution variant (19 in total). The application of GenScript's expertise in de novo gene synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning libaray construction services. |
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The final delivery of our sequential permutation library includes the following:
- Up to 4 ug of mutation constructs
- Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
- Separated variants (optional)
- Sequence verification information
- Statistical analysis of nucleotide distribution at the mutation sites
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3. Randomized and Degenerated Libraries
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With our advanced degenerated oligonucleotide techniques, GenScript can generate any
form of randomization or degeneration of the full-length gene in a synthetic DNA fragment.
This permits controlled, highly precise randomization within oligonucleotides.
GenScript's in vitro library synthesis technology can introduce random substitutions on a controlled level with maximum flexibility. The mutation frequency can be set to any value
between 1 and 20 mutations per kb. A peer group of 48, 96, or 192 individual transformants is sequence-verified.
The randomized and degenerated libraries can be delivered in any of three different formats:
- Non-amplified libraries: Glycerol stock of total non-amplified library of up to 109 transformants
- Amplified libraries: Glycerol stock of amplified transformants or 5 ug of linear DNA ready for cloning
- Cloned libraries (amplified or non-amplified): Glycerol stock of transformants subcloned into customer's vector of choice
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4. Small-scale trial
Please try our service for site-directed mutagenesis at a price starting as low as $295/mutation.
Quotations and Ordering:
 Please send quotation requests via our secure and user-friendly online messaging system or by email. You may also contact us by phone or fax, as listed below.
Orders can be placed by email, phone, or fax with a formal PO (Purchase Order) or credit card. Please submit your DNA/protein sequences, degenerated or mutant positions online using our secure web server. If you prefer, however, you may submit your sequence by email or fax. Our customer service representatives are available 24 hours, Monday through Friday to assist you.
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Order by email: gene@genscript.com |
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Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Order by fax: 1-732-210-0262 1-732-885-5878 |
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