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Mutant Libraries (Protein Engineering)

In vitro molecular optimization is a very efficient means of generating mutant proteins with improved or novel properties, identifying regulatory sequences, and probing for structurally and functionally critical residues. Mutant libraries constructed using the in vitro molecular optimization method provide one useful approach to the systematic study of protein properties, regulation, and function.

GenScript's strong expertise in de novo gene synthesis allows us to synthesize complex protein libraries without any dramatic increase in cost. Our mutant library services include site-directed mutagenesis libraries, sequential permutation scanning libraries, and randomized and degenerated libraries.

Services:

1. Site-Directed Mutagenesis Libraries
Cat. No.: SC1153

GenScript combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. The site-directed mutagenesis library offers a great platform for protein function and active center studies. In these libraries, any given residue can be substituted with any of other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.

The final delivery of our site-directed mutagenesis library includes the following:
  • Up to 10 µg of mutation constructs
  • Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
  • Separated variants
  • Sequence verification information
  • Statistical analysis of nucleotide distribution at the mutation sites
Site-Directed Mutagenesis Libraries
2. Scanning Point Mutation Libraries
Cat. No.: SC1154

Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning by replacing each amino acid with all 20 amino acids simultaneously. This technique provides a detailed profile of each amino acid at the position. For each codon of interest, a small, site-saturated library is constructed. This library can be delivered as a pool or in a separated format for any substitution variant (19 in total). The application of GenScript's expertise in de novo gene synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning library construction services.

The final delivery of our scanning point mutation library includes the following:
  • Up to 10 µg of mutation constructs
  • Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
  • Separated variants (optional)
  • Sequence verification information
  • Statistical analysis of nucleotide distribution at the mutation sites
Sequential Permutation Scanning Libraries
3. Randomized and Degenerated Libraries
Cat. No.: SC1155

With our advanced degenerated oligonucleotide techniques, GenScript can generate any form of randomization or degeneration of the full-length gene in a synthetic DNA fragment. This permits controlled, highly precise randomization within oligonucleotides.

GenScript's in vitro library synthesis technology can introduce random substitutions on a controlled level with maximum flexibility. The mutation frequency can be set to any value between 1 and 20 mutations per kb. A peer group of 48, 96, or 192 individual transformants is sequence-verified.

The randomized and degenerated libraries can be delivered in any of two different formats:
  • Degenerated PCR Products Library (up to 1011 variants) 10 µg of linear DNA ready to clone via 5' and 3' restriction sites.
  • Cloned Library: Library subcloned into customer's vector. Includes glycerol stock of total library transformants. Glycerol stock, up to 109 transformants.
Degenerated Libraries

Quotations and Ordering

  • For quotation requests and questions, you may contact us by phone, email, fax, or via our secured online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.