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 Name
Size   
Price
 Selected
SD1211
 PRNAT-U6.1/Neo
10 μg
$350

pRNAT-U6.1/Neo

Description
pRNAT-U6.1/Neo is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a Neomycin resistance gene as the selectable marker, which can be used for establishing stable cell line. The GFP marker (coral GFP, cGFP) under CMV promoter control can be used to track the transfection efficiency. It uses U6 promoter for siRNA expression.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 78 - 101
U6 Promoter: 6131 - 77
CMV Promoter: 140 - 727
cGFP: 745 - 1463
SV40 Promoter: 2152 - 2497
Neomycin: 2538 - 3332
pUC ori: 4046 - 4686
Ampicillin: 4834 - 5694

Vector Sequence and Restriction Enzyme Map

Limited Use Label License

The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF, at 319-335-4546.

Document

  • Document-PRODUCTINFO: 1125_20051023220029.PDF (PDF)

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