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Cat. No.
 Name
Size   
Price
 Selected
SD1212
 PRNAT-U6.1/Hygro
10 μg
$350

pRNAT-U6.1/Hygro

Description
pRNAT-U6.1/Hygro is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a hygromycin resistance gene as a selectable marker, which can be used for establishing stable cell line. It also has a coral GFP marker (cGFP) under CMV promoter control, which can be used to track the transfection efficiency. It uses a U6 promoter for siRNA expression.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 78 - 101
U6 Promoter: 6300 - 77
CMV Promoter: 140 - 727
cGFP: 744 - 1463
SV40 Promoter: 2152 - 2497
Hygromycin: 2520 - 3545
pUC ori: 4215 - 4855
Ampicillin: 5003 - 5863

Vector Sequence and Restriction Enzyme Map

Limited Use Label License

The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF, at 319-335-4546.

Document

  • Document-PRODUCTINFO: 1127_20051023221302.PDF (PDF)
  • Document-MSDS: 14055_20110926233215.PDF (PDF)

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