One-Step Western^TM Basic Kits

One-Step Western^TM Basic Kits are the simplified version of One-Step Western^TM Complete Kits. The WestClear^TM Nitrocellulose Membrane and LumiSensor^TM Chemiluminescent HRP Substrate Kit and X-Ray film are not included in the package. It is economical to choose these kits if you have surplus accessories in hand. Associated with GenScript's accessories, One-Step Western^TM Basic Kits can perform as excellent as One-Step Western^TM Complete Kits.

To obtain best results and reduce undesirable variations such as high background, we strongly recommend that the following reagents be used with One-Step Western^TM Basic Kit:

• GenScript WestClear^TM Nitrocellulose Membrane (L00224A60, L00224A225).
• GenScript LumiSensor^TM Chemiluminescent HRP Substrate Kit (L00221V60).
• GenScript ChromoSensor^TM One Solution TMB Substrate (L00222V60).

Key Features:

• Easy to perform: Fewer steps mean fewer chances for human error.
• High sensitivity: The kit's sensitivity is comparable to or even better better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
• Reproducible results: The kit produces highly reproducible results.
• Far less optimization than the classical three-step method.
• Secondary antibody is included.

Applications:

• Protein/antigen detection
• Evaluation of protein expression
• Antibody/antigen titration
• Analysis and comparison of primary antibody activity levels

Technical Manual:

Download PDF file TM0352

Product List: