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Western Blotting Kit

ONE-HOUR WesternTM Standard Kit

Developed using our proprietary ONE-HOUR WesternTM technology, ONE-HOUR WesternTM Standard Kit reduces the total analysis time for a Western blot or Dot blot to just one hour. The kit contains all the necessary reagents, buffers, nitrocellulose membrane and HRP substrate for performing western blot, and compatible with both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB.

Key Features:

  • Fast and Easy: The entire western procedure is completed within one hour.
  • Ready-to-Go: The kits come with complete secondary antibodies and need no spedial labeling and far less optimization than traditional three-step kits.
  • High sensitivity: The sensitivity of the ONE-HOUR WesternTM Standard kits is comparable to or better than that of any classical 4.5-hour procedure.

Applications:


ONE-HOUR WesternTM Kit cuts hours off the following applications. ONE-HOUR WesternTM Standard Kit provides everything for such operation:
  • Protein/antigen detection
  • Evaluation of protein expression
  • Antibody/antigen titration
  • Analysis and comparison of primary antibody activity levels

Examples:


Western blot detection of housekeeping protein α-tubulin:
ONE-HOUR Western Standard Kit Western blot detection of α-Tubulin using the ONE-HOUR WesternTM Standard Kit (Cat. No. L00204C) and α-tubulin polyclonal antibody (Abcam, ab4074). 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysates (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit.

Ordering Information:



Cat. No.Name SizePriceQuantity Technical Manual
L00228 ONE-HOUR WesternTM Standard Kit (Goat) 1 Kit (5 Assays) $75.00
ONE-HOUR Western Standard Kit
L00205C ONE-HOUR WesternTM Standard Kit (Mouse) 1 Kit (5 Assays) $75.00
L00204C ONE-HOUR WesternTM Standard Kit (Rabbit) 1 Kit (5 Assays) $75.00
L00204T ONE-HOUR WesternTM Standard Kit Using TMB (Rabbit) 1 Kit (5 Assays) $75.00
L00205T ONE-HOUR WesternTM Standard Kit Using TMB (Mouse) 1 Kit (5 Assays) $75.00
L00228T ONE-HOUR WesternTM Standard Kit Using TMB (Goat) 1 Kit (5 Assays) $75.00