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Western Blot Kit

ONE-HOUR Western™ Detection System

The ONE-HOUR Western™ Detection System is designed to produce high signal with low background for quick and clear western analysis of proteins. GenScript's breakthrough ONE-HOUR Western™ technology simplifies the classical western blot analysis by skipping secondary antibody binding and washing steps. The kits reduce the total western blot analysis time from 4.5 hours down to only one hour.

  • Easy to perform: Quick and simple procedure.
  • High sensitivity: The sensitivity of the ONE-HOUR Western™ is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
  • Highly reproducible results.
  • Less optimization needed than the classical method.
  • Secondary antibody is included.
  • No special labeling required for primary antibody.

Western blot kit

Examples:

A comparison of the ONE-HOUR Western™ Kit to the classical three-step Western procedure is shown below.

western blot kit

After protein transfer from gel to membrane, the users may simply incubate the membrane blot in the ONE-HOUR Pretreatment Solution for five minutes and then in WB Solution with their primary antibody for 40 minutes. We offer a selection of WB Solutions containing secondary antibodies to most commonly used primaries (rabbit, mouse, goat). After three five-minute washes, the membrane is ready to be developed. The entire procedure takes only one hour.

  • Product Details
  • Selection Guide
  • Accessories

ONE-HOUR Western™ Basic Kits

 Cat. No. Name Size Price Quantity
L00204 ONE-HOUR Western™ Basic Kit (Rabbit)
1 Kit (5 Assays)
 $60.00
L00205 ONE-HOUR Western™ Basic Kit (Mouse)
1 Kit (5 Assays)
 $60.00
L00399 ONE-HOUR Western™ Basic Kit (Goat)
1 Kit (5 Assays)
 $60.00

ONE-HOUR Western™ Standard Kits

 Cat. No. Name Size Price Quantity
L00204C ONE-HOUR Western™ Standard Kit (Rabbit)
1 Kit (5 Assays)
 $75.00
L00205C ONE-HOUR Western™ Standard Kit (Mouse)
1 Kit (5 Assays)
 $75.00
L00228 ONE-HOUR Western™ Standard Kit (Goat)
1 Kit (5 Assays)
 $75.00
L00204T ONE-HOUR Western™ Standard Kit with TMB (Rabbit)
1 Kit (5 Assays)
 $75.00
L00205T ONE-HOUR Western™ Standard Kit with TMB (Mouse)
1 Kit (5 Assays)
 $75.00
L00228T ONE-HOUR Western™ Standard Kit with TMB (Goat)
1 Kit (5 Assays)
 $75.00



ONE-HOUR Western™ Advanced Kits

 Cat. No. Name Size Price Quantity
L00241 ONE-HOUR Western™ Advanced Kit (Rabbit)
1 Kit (5 Assays)
 $125.00
L00242 ONE-HOUR Western™ Advanced Kit (Mouse)
1 Kit (5 Assays)
 $125.00
L00243 ONE-HOUR Western™ Advanced Kit (Goat)
1 Kit (5 Assays)
 $125.00



ONE-HOUR IP-Western Kits

 Cat. No. Name Size Price Quantity
L00231 ONE-HOUR IP-Western Kit (Rabbit)
1 Kit (5 Assays)
 $125.00
L00232 ONE-HOUR IP-Western Kit (Mouse)
1 Kit (5 Assays)
 $125.00
L00233 ONE-HOUR IP-Western Kit (Goat)
1 kit (5 Assays)
 $125.00



ONE-HOUR Western™ Fluorescent Kits

 Cat. No. Name Size Price Quantity
L00397 ONE-HOUR Western™ Fluorescent Kit
1 Kit (10 Assays)
 $125.00
L00398 ONE-HOUR Western™ Multiplex Fluorescent Kit
1 Kit (10 Assays)
 $155.00



Western QuickBlock Kit

 Cat. No. Name Size Price Quantity
L00276 Western QuickBlock Kit
1 Kit (10 Assays)
 $60.00

ONE-HOUR Western™ Detection System Selection Guide
ONE-HOUR Series Key Features Total Time Size Price
ONE-HOUR Western™ Basic Kits
  • Easy to perform
  • High sensitivity
  • Reproducible results
  • Use customer own membrane and substrate
 1 hour 1 Kit (5 Assays) $60.00
ONE-HOUR Western™ Standard Kits
  • Recommended for regular users
  • Convenient package with all reagents included
  • Fast and easy to perform
  • High sensitivity and low background
 1 hour 1 Kit (5 Assays) $75.00
ONE-HOUR Western™ Advanced Kits
  • Recommended for detection of protein of low concentration
  • Higher sensitivity and lower background
1.25 hours 1 Kit (5 Assays) $125.00
ONE-HOUR IP-Western Kits
  • For detection of immunoprecipitated proteins
  • Low background
  • Journal-quality images
 1 hour 1 Kit (5 Assays) $125.00
ONE-HOUR Western™ Fluorescent Kit
  • No dark room, developer or X-Ray film is needed
  • More sensitive than traditional chemiluminescence methods
 1.25 hours 1 Kit (10 Assays) $125.00
ONE-HOUR Western™ Multiplex Fluorescent Kit
  • Simultaneous detection of multiple targets on a single blot
  • Saves time associated with optimizing fluorescent Western blots
 1.25 hours 1 Kit (10 Assays) $155.00

Customer Testimonial:

"GenScript Western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent, friendly and efficient."
― Dr. Sarah Yohannan, Yale School of Medicine.


ONE-HOUR Western™ Kit Accessories

GenScript provides a varity of reagents and related products for its line of ONE-HOUR Western™ Kits and ELISA Kits, including detection substrates, solutions and membranes.

Examples:


1. Shown below is a western blot detection of housekeeping gene product GAPDH protein using LumiSensor™ Chemiluminescent HRP Substrate Kit.
Western blot kit Figure 1. 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensor™ Chemiluminescent HRP Substrate Kit.

2. Western blot wash box, dot blot box, and forceps.
western blot forceps, wash box, dot blot box

Ordering Information:


ONE-HOUR Western Detection Substrate

 Cat. No. Name Size Price Quantity
L00221V60 LumiSensor™ Chemiluminescent HRP Substrate Kit
1 Kit (2×30 ml)
 $55.00
L00221V300 LumiSensor™ Chemiluminescent HRP Substrate Kit
1 Kit (2×150 ml)
 $145.00
L00221V500 LumiSensor™ Chemiluminescent HRP Substrate Kit
1 Kit (2×250 ml)
 $235.00
L00222V60 ChromoSensor™ One-Solution TMB Substrate
1 pk (60 ml)
 $28.00
L00222V125 ChromoSensor™ One-Solution TMB Substrate
1 pk (125 ml)
 $39.00
L00222V250 ChromoSensor™ One-Solution TMB Substrate
1 pk (250 ml)
 $68.00
L00222V500 ChromoSensor™ One-Solution TMB Substrate
1 pk (500 ml)
 $119.00


Western Solutions

Cat. No. Name Size Price Quantity
MB01011 5X Wash Solution
125 ml
 $28.00
M01014 A&G Blocker (100 X)
0.5 ml
 $10.00
M01013 Pretreat A Solution (50 ml) & B (50 ml)
1 pack
 $30.00
M01015 Stripping Solution
100 ml
 $29.50


ONE-HOUR Western™ Kit Labware

 Cat. No. Name Size Price Quantity
M00100 Western Wash Box (Clear)
1 pack of 10
 $80.00
M00101 Western Wash Box (Blue)
1 pack of 10
 $80.00
M00102 Western Wash Box (Yellow)
1 pack of 10
 $80.00
M00108 Dot Blot Box (Clear)
1 pack of 10
 $98.00
M00109 Dot Blot Box (Blue)
1 pack of 10
 $98.00
M00110 Dot Blot Box (Yellow)
1 pack of 10
 $98.00
M00099 Western Membrane Forceps
1 pack of 10
 $50.00

WestClear ™ Nitrocellulose Membrane
  Cat. No. Name Size Price Quantity
L00224A60 WestClear™ Nitrocellulose Membrane
1 pk (5 sheets of 7.5 x 8 cm)
 $20.00