The ONE-HOUR WesternTM Kits are a series of new products that cut the total procedure time of a Western or dot blot from 4.5 hours to ONE hour with no loss in sensitivity. GenScript’s scientists are continuously searching for ways to improve technology and reduce costs.
Recently we upgraded to 2nd generation ONE-HOUR Western technology to give you even higher sensitivity and lower background of your Westerns in ONE hour.
Such significant reduction in time is due to the innovative proprietary buffers developed by GenScript, which:
Allow faster blocking.
Promote interaction between primary and secondary antibodies while reducing non-specific binding.
These properties allowed GenScript to reduce blocking time to only five minutes and bypass the separate secondary antibody binding and washing steps.
The combination of GenScript's ONE-HOUR WesternTM Blot Kits with Millipore's SNAP I.D. System* is able to shorten the Western blot analysis time to less than 12 minutes. Technical Manual: ONE-HOUR WesternTM General Guideline Using SNAP I.D.
Simultaneous detection of multiple targets on a single blot
Saves time associated with optimizing fluorescent Western blots
1.25 hours
Customer Testimonial:
"GenScript Western
kits have saved me weeks or months of research time! I absolutely love
them! And the customer service is excellent, friendly and efficient." ― Dr. Sarah Yohannan, Yale School of Medicine
Easy to perform: Quick and simple procedure.
High sensitivity: The sensitivity of the ONE-HOUR WesternTM is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
Highly reproducible results.
Less optimization needed than the classical method.
Secondary antibody is included.
No special labeling required for primary antibody.
A comparison of the ONE-HOUR WesternTM Kit to the classical three-step Western procedure is shown below.
1 hour-1.25hours
ONE-HOUR WesternTM
users may simply transfer proteins from gel to membrane, incubate the
membrane blot in the ONE-HOUR Pretreatment Solution for five minutes
and then in WB Solution with their primary antibody for 40 minutes. We
offer a selection of WB Solutions containing secondary antibodies to
most commonly used primaries (mouse, rabbit, rat, chicken, goat). After
three five-minute washes, the membrane is ready to be developed. The
entire procedure takes only one hour.