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ONE-HOUR WesternTM Blot Kits

2nd generation western

The ONE-HOUR WesternTM Kits are a series of new products that cut the total procedure time of a Western or dot blot from 4.5 hours to ONE hour with no loss in sensitivity. GenScript’s scientists are continuously searching for ways to improve technology and reduce costs. Recently we upgraded to 2nd generation ONE-HOUR Western technology to give you even higher sensitivity and lower background of your Westerns in ONE hour.
Such significant reduction in time is due to the innovative proprietary buffers developed by GenScript, which:

  • Allow faster blocking.
  • Promote interaction between primary and secondary antibodies while reducing non-specific binding.

These properties allowed GenScript to reduce blocking time to only five minutes and bypass the separate secondary antibody binding and washing steps.

The combination of GenScript's ONE-HOUR WesternTM Blot Kits with Millipore's SNAP I.D. System* is able to shorten the Western blot analysis time to less than 12 minutes. Technical Manual: ONE-HOUR WesternTM General Guideline Using SNAP I.D.

western promotion

  • Selection Guide
  • Product list
  • Key Features
  • Examples
ONE-HOUR WesternTM Kit Selection Guide
ONE-HOUR Series Key Features Total Time
ONE-HOUR WesternTM
Complete Kits
  • Recommended for regular users
  • Convenient package with all reagents included
  • Fast and easy to perform
  • High sensitivity and low background
 1 hour
ONE-HOUR WesternTM
Advanced Kits
  • Recommended for detection of low amounts of protein
  • Higher sensitivity and lower background
1.25 hours
ONE-HOUR IP-Western Kits
  • For immunoprecipitated proteins
  • Low background
  • Journal-quality images
 1 hour
ONE-HOUR WesternTM Fluorescent Kit  NEW!
  • No dark room, developer or X-Ray film is needed
  • More sensitive than traditional chemiluminescence methods
 1.25 hours
ONE-HOUR WesternTM Multiplex Fluorescent Kit  NEW!
  • Simultaneous detection of multiple targets on a single blot
  • Saves time associated with optimizing fluorescent Western blots
 1.25 hours

Customer Testimonial:

"GenScript Western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent, friendly and efficient."
                                                                                                                                ― Dr. Sarah Yohannan, Yale School of Medicine

ONE-HOUR WesternTM Complete Kits

 Cat. No. Name Size Price Quantity
L00204T ONE-HOUR WesternTM Kit with TMB (Rabbit) 1 Kit (10 Assays) $255.00
L00205T ONE-HOUR WesternTM Kit with TMB (Mouse) 1 Kit (10 Assays) $255.00
L00228T ONE-HOUR WesternTM Kit with TMB (Goat) 1 Kit (10 Assays) $255.00
L00204C ONE-HOUR WesternTM Complete Kit (Rabbit) 1 Kit (10 Assays) $275.00
L00205C ONE-HOUR WesternTM Complete Kit (Mouse) 1 Kit (10 Assays) $275.00
L00228 ONE-HOUR WesternTM Complete Kit (Goat) 1 Kit (10 Assays) $275.00


ONE-HOUR IP-Western Kits

 Cat. No. Name Size Price Quantity
L00231 ONE-HOUR IP-Western Kit (Rabbit) 1 Kit (10 Assays) $375.00
L00232 ONE-HOUR IP-Western Kit (Mouse) 1 Kit (10 Assays) $375.00
L00233 ONE-HOUR IP-Western Kit (Goat) 1 kit (10 Assays) $375.00


ONE-HOUR WesternTM Basic Kits

 Cat. No. Name Size Price Quantity
L00204 ONE-HOUR WesternTM Basic Kit (Rabbit) 1 Kit (10 Assays) $195.00
L00205 ONE-HOUR WesternTM Basic Kit (Mouse) 1 Kit (10 Assays) $195.00
L00399 ONE-HOUR WesternTM Basic Kit (Goat) 1 Kit (10 Assays) $195.00


ONE-HOUR WesternTM Advanced Kits

 Cat. No. Name Size Price Quantity
L00241 ONE-HOUR WesternTM Advanced Kit (Rabbit) 1 Kit (10 Assays) $345.00
L00242 ONE-HOUR WesternTM Advanced Kit (Mouse) 1 Kit (10 Assays) $345.00
L00243 ONE-HOUR WesternTM Advanced Kit (Goat) 1 Kit (10 Assays) $345.00


ONE-HOUR WesternTM Fluorescent Kit

 Cat. No. Name Size Price Quantity
L00397 ONE-HOUR WesternTM Fluorescent Kit  NEW! 1 Kit (10 Assays) $185.00


ONE-HOUR WesternTM Multiplex Fluorescent Kit

 Cat. No. Name Size Price Quantity
L00398 ONE-HOUR WesternTM Multiplex Fluorescent Kit  NEW! 1 Kit (10 Assays) $245.00


Western QuickBlock Kits

 Cat. No. Name Size Price Quantity
L00276 Western QuickBlock Kit 1 Kit $175.00
L00278 Western QuickBlock Optimization Kit 1 Kit $195.00
L00279 Customized Western QuickBlock Kit A 1 Kit $175.00
L00280 Customized Western QuickBlock Kit B 1 Kit $175.00
L00281 Customized Western QuickBlock Kit C 1 Kit $175.00
L00282 Customized Western QuickBlock Kit D 1 Kit $175.00

  • Easy to perform: Quick and simple procedure.
  • High sensitivity: The sensitivity of the ONE-HOUR WesternTM is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
  • Highly reproducible results.
  • Less optimization needed than the classical method.
  • Secondary antibody is included.
  • No special labeling required for primary antibody.

A comparison of the ONE-HOUR WesternTM Kit to the classical three-step Western procedure is shown below. 1 hour-1.25hours

western blot in one hour

ONE-HOUR WesternTM users may simply transfer proteins from gel to membrane, incubate the membrane blot in the ONE-HOUR Pretreatment Solution for five minutes and then in WB Solution with their primary antibody for 40 minutes. We offer a selection of WB Solutions containing secondary antibodies to most commonly used primaries (mouse, rabbit, rat, chicken, goat). After three five-minute washes, the membrane is ready to be developed. The entire procedure takes only one hour.


FAQs for ONE-HOUR WesternTM Kits.

*SNAP I.D. is a registered trademark of Millipore.