Western Blot Kit

ONE-HOUR Western™ Detection System

The ONE-HOUR Western™ Detection System is designed to produce high signal with low background for quick and clear western analysis of proteins. GenScript's breakthrough ONE-HOUR Western™ technology simplifies the classical western blot analysis by skipping secondary antibody binding and washing steps. The kits reduce the total western blot analysis time from 4.5 hours down to only one hour.

  • Easy to perform: Quick and simple procedure.
  • High sensitivity: The sensitivity of the ONE-HOUR Western™ is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
  • Highly reproducible results.
  • Less optimization needed than the classical method.
  • Secondary antibody is included.
  • No special labeling required for primary antibody.

Western blot kit

Examples

A comparison of the ONE-HOUR Western™ Kit to the classical three-step Western procedure is shown below.

western blot kit

After protein transfer from gel to membrane, the users may simply incubate the membrane blot in the ONE-HOUR Pretreatment Solution for five minutes and then in WB Solution with their primary antibody for 40 minutes. We offer a selection of WB Solutions containing secondary antibodies to most commonly used primaries (rabbit, mouse, goat). After three five-minute washes, the membrane is ready to be developed. The entire procedure takes only one hour.


  • Product Details
  • Selection Guide
  • Accessories

ONE-HOUR Western™ Basic Kits

Cat. No.
Product Name
Size
Price
Quantity
L00204 ONE-HOUR WesternTM Basic Kit (Rabbit)
1 Kit (5 Assays)
$60.00
L00205 ONE-HOUR WesternTM Basic Kit (Mouse)
1 Kit (5 Assays)
$60.00

ONE-HOUR Western™ Standard Kits

Cat. No.
Product Name
Size
Price
Quantity
L00204C-1 ONE-HOUR WesternTM Standard Kit (Rabbit)
1 Kit (5 assays)
$75.00
L00205C-1 ONE-HOUR WesternTM Standard Kit (Mouse)
1 Kit (5 assays)
$75.00
L00204T ONE-HOUR WesternTM Standard Kit Using TMB (Rabbit)
1 Kit (5 Assays)
$75.00
L00205T ONE-HOUR WesternTM Standard Kit Using TMB (Mouse)
1 Kit (5 Assays)
$75.00

ONE-HOUR Western™ Advanced Kits

Cat. No.
Product Name
Size
Price
Quantity
L00241 ONE-HOUR WesternTM Advanced Kit (Rabbit)
1 Kit (5 Assays)
$125.00
L00242 ONE-HOUR WesternTM Advanced Kit (Mouse)
1 Kit (5 Assays)
$125.00

ONE-HOUR Western™ Fluorescent Kits

Cat. No.
Product Name
Size
Price
Quantity
L00397 ONE-HOUR WesternTM Fluorescent Kit
1 Kit (10 Assays)
$125.00
L00398 ONE-HOUR WesternTM Multiplex Fluorescent Kit
1 Kit (10 Assays)
$155.00

Western QuickBlock Kit

Cat. No.
Product Name
Size
Price
Quantity
L00276 Western Quick Block Kit
1 kit
$60.00

LumiSensor™ Chemiluminescent HRP Substrate Kit

Cat. No.
Product Name
Size
Price
Quantity
L00221V60 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 30ml)
$55.00
L00221V300 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 150ml)
$145.00
L00221V500 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 250ml)
$235.00

ChromoSensor™ One-Solution TMB Substrate

Cat. No.
Product Name
Size
Price
Quantity
L00222V60 ChromoSensorTM One-Solution TMB Substrate
1 pk (60ml)
$28.00
L00222V125 ChromoSensorTM One-Solution TMB Substrate
1 pk (125ml)
$39.00
L00222V250 ChromoSensorTM One-Solution TMB Substrate
1 pk (250ml)
$68.00
L00222V500 ChromoSensorTM One-Solution TMB Substrate
1 pk (500ml)
$119.00

Chemiluminescent
Direct View
Components
L00204 Rabbit
L00205
Mouse
L00204C
Rabbit
L00205C
Mouse
L00204T
Rabbit
L00205T Mouse
L00241
Rabbit
L00242
Mouse
Pretreat Solution A (50 ml)
Pretreat Solution B (50 ml)
WB-1 Solution
(0.5 ml)
WB-2 Solution
(50 ml)
5X Wash Solution
(125 ml)
WestClear™ Nitrocellulose Membrane
(0.2 μm, 7.5 x 8.5 cm, 5 sheets)
   
LumiSensor™ Chemiluminescent HRP Substrate
(2 X 7.5 ml)
       
ChromoSensor™ One Solution TMB Substrate
(15 ml)
       
   
SuperSensitive LumiSensor™ Chemiluminescent HRP Substrate
( 5 ml + 10 ml)
           
Dark Room & Film Requirement
**
**
   
User Manual

**Depending on the substrate you use, you need dark room and film if use chemiluminescent HRP substrate, but don't need if you use TMB substrate

Customer Testimonial

"GenScript Western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent, friendly and efficient."
― Dr. Sarah Yohannan, Yale School of Medicine.


ONE-HOUR Western™ Kit Accessories

GenScript provides a varity of reagents and related products for its line of ONE-HOUR Western™ Kits and ELISA Kits, including detection substrates, solutions and membranes.

Examples

1. Shown below is a western blot detection of housekeeping gene product GAPDH protein using LumiSensor™ Chemiluminescent HRP Substrate Kit.
Western blot kit Figure 1. 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensor™ Chemiluminescent HRP Substrate Kit.

2. Western blot wash box, dot blot box, and forceps.
western blot forceps, wash box, dot blot box

Ordering Information


ONE-HOUR Western Detection Substrate

Cat. No.
Product Name
Size
Price
Quantity
L00221V60 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 30ml)
$55.00
L00221V300 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 150ml)
$145.00
L00221V500 LumiSensorTM HRP Substrate Kit
1 Kit (2 X 250ml)
$235.00
L00222V60 ChromoSensorTM One-Solution TMB Substrate
1 pk (60ml)
$28.00
L00222V125 ChromoSensorTM One-Solution TMB Substrate
1 pk (125ml)
$39.00
L00222V250 ChromoSensorTM One-Solution TMB Substrate
1 pk (250ml)
$68.00
L00222V500 ChromoSensorTM One-Solution TMB Substrate
1 pk (500ml)
$119.00


Western Solutions

Cat. No.
Product Name
Size
Price
Quantity
MB01011 5X Wash Solution For One-Step Western Kits
125 ml
$28.00
M01014 A&G Blocker (100X)
0.500 ml
$10.00
M01013 Pretreat A(50 ml) & B(50 ml) Solution
1 pk
$30.00


ONE-HOUR Western™ Kit Labware

Cat. No.
Product Name
Size
Price
Quantity
M00100 Western Wash Box (Clear)
1 pack of 10
$80.00
M00101 Western Wash Box (Blue)
1 pack of 10
$80.00
M00102 Western Wash Box (Yellow)
1 pack of 10
$80.00
M00108 Dot Blot Box (Clear)
1 pack of 10
$98.00
M00109 Dot Blot Box (Blue)
1 pack of 10
$98.00
M00110 Dot Blot Box (Yellow)
1 pack of 10
$98.00
M00099 Western Membrane Forceps
1 pack of 10
$50.00


WestClear™ Nitrocellulose Membrane

Cat. No.
Product Name
Size
Price
Quantity
L00224A60 WestClearTM Nitrocellulose Membrane
1 pk (5 sheets of 7.5 x 8.5 cm)
$20.00