One-Step WesternTM Blot Kit
The One-Step WesternTM Kit is a new product incorporating GenScript's innovative thinking and proprietary technologies.
The One-Step WesternTM cuts total procedure time of a Western or Dot blot from 4½ hours to one hour with no loss in sensitivity.
A classical three-step Western blot requires blocking, primary antibody binding and secondary antibody binding, which can take 4½ hours.
One-Step WesternTM users may simply transfer their proteins from gel to membrane, incubate the membrane in One-Step Pretreatment Solution for five minutes and then in WB solution with primary antibody for 40 minutes, followed by three five-minute washes. Then the membrane is ready to be developed.
Customer Testimonial:
"I would say that Genscript western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent-friendly and efficient." -- Dr. Sarah Yohannan, Yale School of Medicine
Key Features:
- Easy to perform: Quick and simple procedure.
- High sensitivity: The sensitivity of the One-Step WesternTM is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
- Highly reproducible results.
- Needs less optimization than the classical method.
- No secondary antibody needed.
- No special labeling required for primary antibody.
Western Blot Kit Selection Guide
For best results, we highly recommend the complete kits, which include both membrane and reagents for blot development. The complete kits eliminate variability from membrane and development reagents.
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