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ERK1/2 Antibody (Phospho-Thr202/Tyr204), pAb, Rabbit

*This product has been discontinued!*
ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases.GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic dual phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482).
A01386
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Overview
Specificity GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody detects endogenous levels of human and rat ERK1 and ERK2 when dually phosphorylated at Thr202 and Tyr204 of ERK1 (Thr185 and Tyr187 of ERK2), and singly phosphorylated at Tyr204. The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase. Predicted to react with mouse ERK1 and ERK2 protein according to sequence homology.
Host Species Rabbit
Immunogen KLH-coupled synthetic dual phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482)
Species Reactivity Human and rat. This product has not been tested with other species yet.
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Properties
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Purification Affinity chromatography using dual phosphopeptide, cross -adsorbed with corresponding single phosphopetide and non-phosphopeptide
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Background
Target Background ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases.

GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic dual phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482).
Synonyms Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) pAb;
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