ERK1/2 Antibody (Phospho-Thr202/Tyr204), pAb, Rabbit
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ERK1/2 Antibody (Phospho-Thr 202/Tyr 204), pAb, Rabbit  |
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| Full Name |
ERK1/2 Antibody (Phospho-Thr202/Tyr204), pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) pAb |
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Description |
ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases.
GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic dual phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482). |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-ERK1/2 (Phospho-Thr202/Tyr204) Polyclonal Antibody detects endogenous levels of human and rat ERK1 and ERK2 when dually phosphorylated at Thr202 and Tyr204 of ERK1 (Thr185 and Tyr187 of ERK2), and singly phosphorylated at Tyr204. The antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase. Predicted to react with mouse ERK1 and ERK2 protein according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic dual phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human ERK1 (Swiss Prot: P27361) and ERK2 (Swiss Prot: P28482) |
Purification Method |
Affinity chromatography using dual phosphopeptide, cross -adsorbed with corresponding single phosphopetide and non-phosphopeptide
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Reconstitution |
Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Other Applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
MSDS: 20090427230825 (PDF)
