| Product Name |
CHO-K1/EDNRA Stable Cell Line |
Full Name |
Human Recombinant Endothelin Receptor Type A Stable Cell Line |
|
Species |
Human |
Description |
In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRA receptors are mainly localized in the vascular smooth muscle cells, where they are the predominant sub-type, and mediate vasoconstriction and proliferation. Selective EDNRA antagonists are effective in the treatment of heart failure, essential hypertension, pulmonary hypertension, and atherosclerosis. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for EDNRA receptor |
Application Examples |
Figure 1. ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRA and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRA receptor agonist, ET-1. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of ET-1 (Mean ± SD, n = 2). The EC50 of ET-1 on EDNRA in CHO-K1 cells was 3.9 nM. The S/B of ET-1 on EDNRA in CHO-K1 cells was 21. |
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