| Product Name |
CHO-K1/EDNRB Stable Cell Line |
Full Name |
Human Recombinant Endothelin Receptor Type B Stable Cell Line |
|
Species |
Human |
Description |
In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRB receptors present on the endothelial cells lining the vessel wall and play a role in the release of endothelium-derived relaxing factors such as nitric oxide (NO) and prostanoids and in removing ET from the circulation. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for EDNRB receptor |
Application Examples |
Figure 1. ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist, ET-1. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of ET-1 (Mean ± SD, n = 2). The EC50 of ET-1 on EDNRB in CHO-K1 cells was 4.3 nM. The S/B of ET-1 on EDNRB in CHO-K1 cells was 35. |
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