Catalog Products » Other Products » Stable Cell Lines » Human Recombinant D2 Dopamine Receptor Stable Cell Line
Email to GenScript

Cat. No.
Product Name
M00152 CHO-K1/D2/Gα15 Stable Cell Line
2 vials
Product Name CHO-K1/D2/Gα15 Stable Cell Line
CHO-K1/D2/Gα15 Stable Cell LineTECHNICAL MANUAL: 9720_20090503223310.PDF (PDF)
CHO-K1/D2/Gα15 Stable Cell LineDocument-MSDS: 10373_20090813060542.PDF (PDF)
CHO-K1/D2/Gα15 Stable Cell Line zoomCHO-K1/D2/Gα15 Stable Cell Line
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). Dopamine receptor 2 is mainly expressed in the brain. It has splicing variants, D2L and D2S. D2R receptor is implicated in a number of neurological and psychiatric conditions. Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson's disease, schizophrenia, and depression.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin,100 μg/ml Hygromycin B
Liquid nitrogen immediately upon delivery
Functional assays for D2 receptor
Application Examples
Figure 1. Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, Dopamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Dopamine (Mean ± SD, n = 2). The EC50 of Dopamine on D2 co-expressing with Gα15 in CHO-K1 cells was 30 nM. The S/B of Dopamine on D2 in CHO-K1 cells was 18.
Related Products
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.