| Product Name |
CHO-K1/D2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant D2 Dopamine Receptor Stable Cell Line |
|
Species |
Human |
Description |
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). Dopamine receptor 2 is mainly expressed in the brain. It has splicing variants, D2L and D2S. D2R receptor is implicated in a number of neurological and psychiatric conditions. Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson's disease, schizophrenia, and depression. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin,100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for D2 receptor |
Application Examples |
Figure 1. Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, Dopamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Dopamine (Mean ± SD, n = 2). The EC50 of Dopamine on D2 co-expressing with Gα15 in CHO-K1 cells was 30 nM. The S/B of Dopamine on D2 in CHO-K1 cells was 18. |
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TECHNICAL MANUAL: 9720_20090503223310.PDF (PDF)
Document-EXAMPLE: 9725_20090503223616.JPG (JPG)