| Product Name |
CHO-K1/CCKB Stable Cell Line |
Full Name |
Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Cholecystokinins (CCK) are derived from preprocholecystokinin. They are widely expressed in gastrointestinal tissues and in the CNS. There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). CCKBR can be activated by the smaller CCK-4 (7, 8). In the CNS, CCKBR has been implicated in anxiety, depression, schizophrenia, depression, and opioid analgesia. In the stomach, CCK2 mediates gastrin-stimulated gastric acid secretion. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CCKB receptor |
Application Examples |
Figure 1. CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCK8 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a CCKB receptor agonist, CCK8. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of CCK8 (Mean ± SD, n = 2). The EC50 of CCK8 on CCKB in CHO-K1 cells was 0.72 nM. The S/B of CCK8 on CCKB in CHO-K1 cells was 70. |
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TECHNICAL MANUAL: 10002_20090629224946.PDF (PDF)
Document-EXAMPLE: 10004_20090629225322.JPG (JPG)