| Product Name |
293/FP/Gα15 Stable Cell Line |
Full Name |
Human Recombinant FP Prostanoid Receptor Stable Cell Line |
|
Species |
Human |
Description |
Prostaglandin F (2-alpha) is known as a potent luteolytic agent. It is involved in modulating intraocular pressure and smooth muscle contraction in uterus. Its effects on cells are mediated through specific interaction with the Prostanoid receptor FP, which is a 359-amino acid protein. Having 7 putative transmembrane domains, FP resembles the characteristic of G protein coupled receptors. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thereupon induce luteolysis. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 200 μg/ml Zeocin, 250 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for FP receptor |
Application Examples |
Figure 1. Cloprostenol-induced concentration-dependent stimulation of intracellular calcium mobilization in 293/FP/Gα15 and 293 cells. The cells were loaded with Calcium-4 prior to stimulation with an FP receptor agonist, Cloprostenol. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Cloprostenol (Mean ± SD, n = 2). The EC50 of Cloprostenol on FP co-expressing with Gα15 in 293 cells was 25 nM. The S/B of Cloprostenol on FP co-expressing with Gα15 in 293 cells was 31. |
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
|
Document-EXAMPLE: 9778_20090520044926.JPG (JPG)
TECHNICAL MANUAL: 9835_20090524225814.PDF (PDF)